LGC publishes new guide on practical laboratory skills for molecular biologists


This new guide is intended to support those working in a molecular biology laboratory.

Tim Wilkes, researcher in LGC’s Molecular Biology team, and co-author of the guide, explains, “An analyst working in any kind of laboratory will require a basic set of practical skills, but molecular biology laboratories provide scenarios and analytical materials which demand specific practical considerations.”

This new best practice guide covers the skills and other measures required to produce valid results in a molecular biology laboratory − results that are not compromised by poor practical technique, possible cross-contamination or non-validated methodology.

There are sections on basic practices, such as measuring mass or volume, and preparing solutions of known concentration. Additional skills that relate to the nature of the materials being handled in a molecular biology laboratory, such as preventing contamination of the samples and protecting the analyst, critical concerns for molecular biologists, are also detailed.

Practical laboratory skills for molecular biologists is freely available for download.

This guide has been produced with support from the UK National Measurement System, funded by the Department for Business, Innovation and Skills.

Use of microsatellite markers for the assessment of bambara groundnut breeding system and varietal purity before genome sequencing


ABSTRACT

Maximizing the research output from a limited investment is often the major challenge for minor and underutilized crops. However, such crops may be tolerant to biotic and abiotic stresses and are adapted to local, marginal, and low-input environments. Their development through breeding will provide an important resource for future agricultural system resilience and diversification in the context of changing climates and the need to achieve food security. The African Orphan Crops Consortium recognizes the values of genomic resources in facilitating the improvement of such crops. Prior to beginning genome sequencing there is a need for an assessment of line varietal purity and to estimate any residual heterozygosity. Here we present an example from bambara groundnut (Vigna subterranea (L.) Verdc.), an underutilized drought tolerant African legume. Two released varieties from Zimbabwe, identified as potential genotypes for whole genome sequencing (WGS), were genotyped with 20 species-specific SSR markers. The results indicate that the cultivars are actually a mix of related inbred genotypes, and the analysis allowed a strategy of single plant selection to be used to generate non-heterogeneous DNA for WGS. The markers also confirmed very low levels of heterozygosity within individual plants. The application of a pre-screen using co-dominant microsatellite markers is expected to substantially improve the genome assembly, compared to a cultivar bulking approach that could have been adopted.

Ho WK, Muchugi A, Muthemba S, Kariba R, Mavenkeni BO, Hendre P, Song B, Deynze AV, Massawe F, Mayes S. Genome (2016) Use of microsatellite markers for the assessment of bambara groundnut breeding system and varietal purity before genome sequencing. 59: 427-431, DOI: 10.1139/gen-2016-0029. http://www.nrcresearchpress.com/doi/abs/10.1139/gen-2016-0029?src=recsys&journalCode=gen#.V-kutSF96M8

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